Plasmid

Part:BBa_K4204012

Designed by: Chujing Wu   Group: iGEM22_BNDS_China   (2022-10-06)


IPTG + sfGFP + Protein quality control system

The protein quality control system (Pro-QC system) is universal that can be applied to all kinds of protein expression. Since Lac operon is the most common induction system used for protein expression, our team improved upon it and uploaded the Protein quality control system with GFP between the switch and trigger on pET-28a(+) (a common expression vector with His-tag and Lac operator) to the part registry (in which the GFP could be replaced by other parts); the western blot result of LysPBC5 with the protein quality control system shows the system's efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 973
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 105
    Illegal NheI site found at 138
    Illegal NheI site found at 161
    Illegal SpeI site found at 973
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 973
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 973
    Illegal AgeI site found at 346
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4
    Illegal BsaI.rc site found at 1047


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Categories
Parameters
None